Research Technologist (Ophthalmology)

We are seeking a Research Technologist who will perform laboratory tasks and completes routine assays following common or established protocols and procedures. We are seeking a highly motivated, energetic individual with an interest in biomedical research to work in the field of Retinal Regenerative Medicine. We use stem cell biology to study human neuronal and glial cells. We use CRISPR genome editing to develop models of 1) human retinal diseases, with a focus on neurodegenerative diseases such as glaucoma, 2) demyelinating diseases (such as multiple sclerosis where myelin sheath on the CNS neurons is lost), 3) Neurofibromatosis 1 (NF1), with a focus on the genetic mutations in NF1 gene that cause dysfunction of glial cells such as astroglia, oligodendroglia and microglia. We also use mouse model of neurodegenerative diseases for drug testing.This work will involve a considerable amount of cell culture and molecular biology work. The successful applicant will work under the supervision of the principal investigator and other laboratory personnel and will be responsible for maintaining human pluripotent stem cells, as well as differentiating them into human retinal ganglion cells, oligodendrocytes, astrocytes, and microglia. They will also assist in various molecular biology work that include molecular cloning, DNA/RNA extraction, PCR, qPCR, gel electrophoresis, and Western Blotting etc. While the majority of the work is related to cell culture and molecular biology, some animal handling and tissue processing will also be required, which they will need to perform under the supervision of the principal investigator or a senior scientist.Specific Duties & ResponsibilitiesPerform clinical and/or research laboratory experiments by following common protocols independently or with instructions. Use basic science knowledge to learn techniques and methods of the lab. Gain problem-solving skills through progressive training, understanding of the research, and experience. Run lab tests and complete measurements. Identify and resolve common problems involved in routine assays. Increase skill base and knowledge of lab research and relevant assays. Train users in standard equipment operation and routine lab techniques and procedures. Set-up experiments based on defined standards. Collect project data. Prepare, test, and maintain standard laboratory equipment. Perform related laboratory maintenance, monitor inventory of supplies, clean equipment. Other duties as assigned.Minimum QualificationsBachelor’s Degree in Biology, Chemistry or related field.Additional education may substitute for required experience and additional related experience may substitute for required education beyond a high school diploma/graduation equivalent, to the extent permitted by the JHU equivalency formula.Preferred QualificationsSome relevant post-undergraduate work experience in the field of cellular and molecular biology is desirable. Experience with cell culture work, ideally with human stem cell culture, is desirable. Prior experience handling and working with animal models is a plus.Classified Title: Research Technologist Role/Level/Range: ACRO40/E/02/CC Starting Salary Range: $16.10 - $26.90 HRLY ($49,140 targeted; Commensurate w/exp.) Employee group: Full Time Schedule: Monday-Friday, 8:30 am - 5:00 pm FLSA Status: Non-Exempt Location: School of Medicine Campus Department name: SOM Oph Ocular Molecular Biology Personnel area: School of Medicine Department:Research