Research Professional

Position Summary The Research Professional has duties to design, develop, organize, and supervise the work on a major recombinant protein expression project in the lab employing bacterial or mammalian cell culture systems. The goals of this research project will provide large‑scale protein production for enzyme structural and functional studies. The position entails establishing project goals, working directly with supervising faculty to design and implement new recombinant constructs, developing priorities among the large number of constructs, generating and large‐scale producing recombinant DNAs, and supervising the work of other research technicians to accomplish these goals. Work duties include close collaboration with senior faculty and working within a larger research group of postdoctoral researchers, research technicians, and undergraduate researchers to develop strategies for high‑quantity protein expression, and close interaction with investigators at other research sites for the design and generation of new constructs for collaborative studies. Additional roles involve supervision of research technicians and undergraduate researchers on preparation of plasmid DNAs from bacterial cultures, executing protocols for novel transfection strategies of mammalian cells, and training research technicians and undergraduate researchers on standard operating procedures. Direct supervision of the Research Professional by senior faculty is anticipated. Qualifications Minimum: Requires at least a baccalaureate degree in the field. Please contact your Human Resources office for an evaluation of education/experience in lieu of the required minimum qualifications. Preferred: A college degree in science, laboratory and computer experience is necessary (a Master’s degree is preferred), as well as experience in molecular biology, protein expression, purification, and general laboratory methods are required. The job requires detailed capabilities to carry out molecular biology and biochemistry protocols, run all aspects of the expression platform as well as effective capabilities for coordinating and supervising numerous concurrent projects from initiation to completion. The expectation is that the employee will be well trained in molecular biology, protein biochemistry, and enzymology, with a preferred expertise with bacterial propagation and mammalian cell culture. Experience in molecular biology, protein expression, purification, and general laboratory methods are required. Responsibilities Design new recombinant DNA constructs within the Moremen lab and interaction and design of constructs in consultation with researchers at remote sites for collaborative projects (35%). Generation of recombinant constructs and verification by DNA sequencing (15%). Large scale isolation of recombinant DNAs for mammalian cell transfection (25%). Identifying optimal protocols for isolation of large‑scale plasmid DNA preparations (5%). Establishing and optimizing strategies and protocols for purification of expressed proteins (10%). Training and supervision of Research Technicians and undergraduate researchers in their duties on generation of large‑scale plasmid preparations of recombinant constructs, protein purification, and characterization. Interaction with structural biology groups for preparation of samples for enzymatic glycan synthesis, NMR analysis, and crystallization. Contact Recruitment Contact: Kelley Moremen Email: View email address on click.appcast.io Equal Employment Opportunity The University of Georgia is an Equal Opportunity employer. All qualified applicants will receive consideration for employment without regard to age, color, disability, genetic information, national origin, race, religion, sex, or veteran status or other protected status. Persons needing accommodations or assistance with the accessibility of materials related to this search are encouraged to contact Central HR (View email address on click.appcast.io). #J-18808-Ljbffr