Research Technician

We are particularly looking for a motivated, energetic, flexible, organized, and independent individual. Key activities will include CRISPR-based genetic engineering and culturing of adherent mammalian cell lines, biochemical purification and characterization of extracellular vesicles (EVs), mouse tissue harvesting and processing, Western blotting, and other experimental techniques in support of ongoing research projects. The successful applicant will have demonstrated proficiency with adherent cell culture. Experience with biochemical isolation and purification is preferred. The Research Technician will work alongside other research staff in the laboratory to facilitate ordering, equipment maintenance and training, and laboratory organization. This is a full‑time, hourly position with very limited weekend hours that may be required on occasion. The applicant will report directly to Dr. Alissa Weaver and work closely with senior members of the laboratory. The Research Technician will be part of the Weaver laboratory in the Cell and Developmental Biology Department at Vanderbilt University. Responsibilities include extracellular vesicle purifications and quality control analysis, development of new cell lines for cancer‑related EV studies, and sample processing for downstream analyses (imaging, proteomics, lipidomics, RNA sequencing). Much of the work relates to a project on how RNA is trafficked into EVs and how those RNA‑containing EVs drive colon cancer aggressiveness. Key Functions and Expected Performance Extracellular Vesicle purification and analysis Adherent cell culture Genetic engineering of cell lines Carrying out experiments under supervision Proactively troubleshooting and seeking out advice/feedback Maintaining accurate experimental records Meeting at least once a week with Dr. Weaver and/or laboratory subgroups to present data and report on the week’s activities Maintaining equipment and reagent stocks Organizing laboratory protocols in shared online databases Qualifications A bachelor’s degree in biology, chemistry, or a related field of study from an accredited institution of higher education is required. Experience performing adherent mammalian cell culture is required. Experience performing biochemical purification is preferred. At least one consecutive year of related laboratory experience is preferred, either in undergraduate research or in a post‑graduate technical position. Strong organizational, oral, and written communication skills are required. Supervisory Relationships This position does not have supervisory responsibility; it reports administratively and functionally to the Principal Investigator of the laboratory, Dr. Alissa Weaver. Vanderbilt University is an equal‑opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran, or any other characteristic protected by law. #J-18808-Ljbffr