High-Throughput Screening Research Associate II, III (Biodesigner II, III) (San Jose) at Amber [...]

Amber Bio is a biotechnology company pioneering new gene editing modalities using multi‑kilobase edits to reach previously undruggable patient populations. Founded by pioneers in the CRISPR field from leading institutions for gene editing research, the company is developing a first‑of‑its‑kind RNA editing platform that can correct thousands of bases at once, thereby correcting genetic mutations safely and reversibly. If you are interested in building a new frontier in genetic medicine, we welcome you to apply. Job Description: High-Throughput Screening Research Associate II, III (Biodesigner II, III) Responsibilities Perform massively parallel reporter assays and high‑throughput screens across diverse cellular contexts using cellular and molecular readouts. Develop and execute molecular biology workflows such as vector design and cloning, DNA/RNA extraction, RT‑PCR, qPCR, and next‑generation sequencing. Support cell culture activities and experiments in multiple cell lines, at small and large scales. Design and execute cell‑based assays (AAV/lentiviral transduction, transfection, flow cytometry, immunostaining, and other plate reader assays). Engineer and characterize cell‑based systems using synthetic biology tools and techniques. Conduct and troubleshoot experiments, independently and in collaboration with colleagues, to optimize screening throughput, sensitivity, and specificity. Proactively troubleshoot technical issues and recommend potential corrective actions based on personal observations and literature searches. Prepare summaries of data and present internally to colleagues and management. Draft SOPs, follow protocols, diligently document experimental data in lab notebooks, and organize and maintain electronic work records. Author scientific reports and data summaries. Collaborate with cross‑functional teams to meet project goals, bridging early discovery with high‑throughput screens to nominate and optimize candidates for further characterization. Qualifications Bachelors or Masters degree in Biology, Biochemistry, Chemical Engineering, Biological Engineering, or a related field. At least 2 years of industry wet lab experience. Mammalian cell culture experience (culturing, transfecting and transducing cells, and DNA/RNA purification from cells). Molecular biology expertise (vector design and cloning, qPCR, primer and probe design, DNA/RNA extraction workflows) Critical thinker with excellent communication skills who thrives in a multidisciplinary, fast‑paced team environment. Strong written and verbal communication skills. Preference will be given to those who display: High throughput screening assay development in an industry setting. High motivation, with a strong work ethic and dedication to generating impact. Attention to detail, with the ability to extract deep insights from data. First‑principles thinking, and an ability to refine one's intuition based on additional data. Ability to go from ideation to data in an independent fashion. Long‑term personal vision with defined career goals. High EQ with team‑oriented thinking. Experience with pooled, high‑throughput screens using next‑generation sequencing‑based readouts, and/or preparing screening plasmid libraries from synthesized oligo arrays. Experience with CRISPR‑Cas systems and/or gene editing and delivery technologies. Experience preparing next‑generation sequencing libraries (Illumina, PacBio, and/or Nanopore platforms). Amber Bio is an equal‑opportunity employer and encourages applications from candidates of diverse backgrounds. We value diversity and are committed to creating an inclusive and supportive work environment for all employees. #J-18808-Ljbffr Downtown Boulder Partnership