Senior Research Technician - Molecular Microbiology

Scheduled Hours 40 Position Summary The Kanjee lab studies how malaria parasites infect human red blood cells (RBCs). We are interested in identifying host receptors required by the parasite during RBC invasion, with a specific focus on the understudied parasite Plasmodium vivax. We use red blood cell genetics approaches (CRISPR/Cas9 knockout/knockdown & gene complementation) to understand the functional mechanisms of candidate host receptors during parasite invasion. This position in the Kanjee lab will assist with experiments involving general molecular cloning, designing CRISPR/Cas9 knockout, knockdown and host receptor gene complementation constructs, in vitro culturing of primary and immortalized erythroid cells, culturing of malaria parasites, validation of host RBC genetic knockouts/knockdowns/complementation via flow cytometry and Western blot. These experiments will involve: collection of data, preparation of cultures, media and set-up and maintenance of equipment. Performs independently complex research studies, experiments and assays. Primary Duties & Responsibilities Performs complex analysis/projects according to research protocols, explaining methods and procedures to other technicians. Enters data and maintains current data files on research projects. Monitors data to ensure consistent quality. Prepares tissue cultures, reagents, probe tests, etc. May perform preliminary statistical analysis of data. Assures compliance with established safety procedures and maintains required documentation on laboratory and specimen conditions. Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures. Assists in the training and development of technicians, acting as a group leader. Performs other duties as assigned. Working Conditions Works in a laboratory environment with potential exposure to biological and chemical hazards. Must be physically able to wear protective equipment and to provide standard care to research animals. Required Qualifications Education: Master’s degree or combination of education and/or experience may substitute for minimum education. Certifications/Professional Licenses: No specific certification/professional license is required for this position. Work Experience: Laboratory Setting (1 Year) Skills: Not Applicable Driver's License: A driver's license is not required for this position. Preferred Qualifications Prior experience with in vitro cell culture in BSL2 work environment is an asset. Prior experience with mammalian genetics approaches and molecular cloning is an asset. Prior experience with parasite culture is an asset. Working knowledge of spreadsheets and statistical analyses. Excellent record keeping, organizational skills, and ability to interact with others. Grade R08-H Salary Range $18.77 - $31.94 / Hourly EEO Statement Washington University in St. Louis is committed to the principles and practices of equal employment opportunity. It is the University’s policy to provide equal opportunity and access to persons in all job titles without regard to race, ethnicity, color, national origin, citizenship (where prohibited by federal law), age, religion, sex, sexual orientation, gender identity or expression, disability, protected veteran status, or genetic information. #J-18808-Ljbffr Washington University in St. Louis