Senior Associate Scientist II

Company Overview: Prime Medicine is a leading biotechnology company dedicated to creating and delivering the next generation of gene editing therapies to patients. The Company is leveraging its proprietary Prime Editing platform, a versatile, precise and efficient gene editing technology, to develop a new class of differentiated, one-time, potentially curative genetic therapies. Designed to make only the right edit at the right position within a gene while minimizing unwanted DNA modifications, Prime Editors have the potential to repair almost all types of genetic mutations and work in many different tissues, organs and cell types. Prime Medicine is currently progressing a diversified portfolio of investigational therapeutic programs organized around our core areas of focus: hematology, immunology & oncology, liver and lung. Across each core area, Prime Medicine is focused initially on a set of high value programs, each targeting a disease with well-understood biology and a clearly defined clinical development and regulatory path, and each expected to provide the foundation for expansion into additional opportunities. For more information, please visit Duties: Manage database and serve as primary liaison for mRNA requests across Prime’s research and program teams. Responsible for independently executing mRNA production at various scales; from high-throughput plate-based scale for screening using automation, to lab scale, to production of clinically-representative GLP toxicology batches, and serve as Person in Plant for GMP manufacturing to provide technical oversight. Contribute to both guide RNA production at various sites and scales (research, process scale and GMP) as needed. Collaborate with Analytical and QC teams to align testing and release workflows, ensuring efficient material readiness. Track and communicate batch status, material availability, and testing timelines to stakeholders. Execute critical experiments and batch runs to generate IND/BLA supporting data for liver-targeted and partnered programs. Conduct statistical and advanced analytics analyses of experimental data for process and product knowledge, producing graphs for CMC technical reports and IND/BLA filings. Plan and execute implementation of process improvements and cost-saving initiatives, including raw material evaluation. Perform in vitro transcription (IVT), capping, and purification operations, including AKTA-based chromatography. Design and support the development of analytical methods. Apply DOE principles to optimize reaction conditions and interpret experimental outcomes using advanced analytics. Operate automated systems for plate-based mRNA production and sample processing (e.g., Lynx, Big Tuna, ResolveX, Tecan, and Hamilton). Train peers on automated platforms and contribute to expanding throughput capabilities across Prime’s teams. Requirements: Bachelor’s Degree in Biochemistry, Chemistry, Chemical Engineering, Biomedical Engineering, or related field or foreign degree equivalent and 5 years of experience working on drug substance process development. Required Skills: 2 years of experience with analytical assay development. 4 years of experience with process development of enzymatic reactions for mRNA production, specifically with the use of statistical design of experiments (DoE) and analysis. 5 years of experience in downstream processing with special emphasis on chromatography of biologics and RNA (using AKTA or similar, as well as scaled down, automated systems). 1 year of experience with the synthesis and purification of long guide oligonucleotide RNA molecules. 3 years of experience with high throughput/automation development, programming, and execution (including Tecan, Hamilton, Lynx, or similar). 4 years of experience utilizing analytical characterization techniques, such as: HPLC/UPLC, TEM, DSC, UV-Vis, fluorescence spectrophotometry, and plate-based ELISA assays to support process development. 5 years of experience capturing, documenting, and maintaining records of research findings, experimental planning, progress and results in a technically-accurate, scientifically-integral and understandable manner. 2 years of experience with the Generation of manufacturing batch documents, and analytical methods development SOPs, and preparation of technical reports for IND/CTA submissions. Salary: $103,500/year – $115,000/year Resumes to: Human Resources, View email address on click.appcast.io. The pay range reflects the full-time base salary range we expect to pay for this role at the time of posting. Base pay will be determined based on several factors including, but not limited to, relevant experience, skills, and education. This role is eligible for an annual short-term incentive award (e.g., bonus) and an annual long-term incentive award (e.g., equity). Prime Medicine offers a comprehensive Total Rewards package with robust health, financial, and wellness benefits—including medical, dental, vision, life insurance, a 401(k) match, and equity programs—along with generous paid time off, wellness days, and company-wide recharge breaks. Our commitment to employee well-being reflects our belief that when we care for our people, we strengthen our ability to put patients first and make meaningful impact together. U.S. Pay Range

$103,500—$115,000 USD

Prime Medicine is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, age, sexual orientation, gender identity, disability, veteran status, or any other classification protected by law.