Research Technician II

Research Technician II

Work Arrangement: On-Site

Location: Durham, NC, US, 27710

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Be You. The Dou laboratory at Duke University is seeking a highly-motivated and qualified individual for the position of Research Technician II to assist in setting up a cutting-edge RNA biology and sequencing lab geared towards defining the mechanisms and drivers for autoimmune diseases. The Dou lab utilizes a multidisciplinary approach including biochemical assays, tissue culture, mouse models, FACS/flow cytometry, and high throughput sequencing among other assays to study the underlying causes and developmental course of autoimmune diseases from a lncRNA and epigenetic gene regulation perspective. This research technician position involves both experimental research and lab management responsibilities. The successful candidate will work both independently and under direct supervision from the principal investigator as necessary. The technician will be directed as to which projects to pursue, methods to employ, and receive any necessary training for new techniques, but will be expected to complete a number of experiments and day-to-day monitoring of mice without requiring immediate supervision.

Minimum Requirements:

• B.S. or B.A. in basic biosciences-related major
• At least one year of related laboratory work

Preferred Qualifications:

• Animal handling experience preferred
• A postgraduate degree (e.g., Master of Science, etc.) is a valuable plus

Be Bold.

• Perform routine lab maintenance, cleaning, ordering and organizing
• Understand, comply and assist in enforcing all safety standards and procedures to ensure a safe working environment for all laboratory staff
• Maintain adequate stock of lab supplies and ensure proper setup and functioning of equipment
• Manage a transgenic mouse colony, including but not limited to: live mouse handling, ordering, genotyping, breeding, routine monitoring of animal health, sample collection and processing, maintaining and updating mouse line records on multiple databases, and etc.
• Perform simple techniques in cell biology and microscopy: examples include cell isolation and culture procedures, immunocytochemistry, tissue cryosectioning, microscope imaging, cell sorting, and some basic molecular biology and biochemistry.
• Perform complex experiments working with both animal and human specimens in teams and individually: examples include sample preparation for storage and downstream studies, protein/nucleic acid isolation, immunoprecipitation, sequencing library and proteomics workflows, and etc.
• Utilize standard protocols through the application of theoretical knowledge acquired from input from supervisor and experiments in the field, in order to simplify or reduce error and inconsistency in results.
• Assist in the design and analysis of experiments; carry out experiments with minimal or no immediate supervision and record and maintain all acquired data in specified formats for scientific reliability and general comprehension
• Learn and develop new lab techniques and assist in the training of new personnel.
• Perform other duties as requested by the Principal Investigator

Choose Duke. This position is geared for the highly motivated individual with a preferred background in RNA biology and/or immunology and wet lab experience. Successful candidates for this position must display self-initiative and motivation, teamwork, excellent communication, organizational, and problem-solving skills. Preference will be given to applicants with animal handling experience. Some basic computer science background (python or R programming) would be useful.

Anticipated Pay Range: Duke University provides an annual base salary range for this position as USD $19.06 to USD $28.85. Duke University considers factors such as (but not limited to) scope and responsibilities of the position; candidate's work experience, education/training, and key skills; internal peer equity; as well as market and organizational considerations when extending an offer. Your total compensation goes beyond the dollars on your paycheck. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array of family-friendly and cultural programs to eligible team members. Learn more at: Equal Opportunity Employer: Duke is an Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, (including pregnancy and pregnancy related conditions), sexual orientation, or military status. Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas—an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values. Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and reasonable accommodation(s) can be requested with Duke Access and Accommodations Services (email: View email address on click.appcast.io; phone: View phone number on click.appcast.io).