Staff Research Associate (9613C), Molecular & Cell Biology - 86302

Position Summary The UC Berkeley DNA Sequencing Facility is one of the scientific recharge facilities managed by the Molecular and Cell Biology Department. The facility supports research by providing Sanger sequencing, full plasmid sequencing (NGS), genotyping, high‑throughput nucleic acid purification/quantification, PCR cleanup, PCR product size selection, human cell line authentication, stem cell authentication, and other DNA‑related services. We also provide open‑access instruments and often free consultation services to roughly 200 research labs on and off campus. The facility recharges approximately $1,000,000 annually and employs the director and several staff research associates. Approximately 10,000–15,000 DNA sequencing requests are processed each month for our diverse customer base. Job Scope The associate will work with the director, SRAs, and undergraduate laboratory assistants and will assist in many daily operations and upkeep of the DNA sequencing facility. Responsibilities include assisting with cycle sequencing reaction assembly, post‑cycle sequencing cleanup, plasmid purification, primer dilutions, solution preparations, PCR cleanup, nucleic acid quantification, data analysis, and data entry. After gaining competence with routine tasks, the associate will advise researchers on sample preparation and support the director with Nanopore and other next‑generation sequencing activities. Responsibilities DNA Sample Processing & Sequencing Operations (50%) Assist with daily opening of the facility and daily protocols for DNA sample processing under supervision. Complete final steps of sample processing initiated the evening prior. Prepare incoming DNA sequencing samples and set up reactions for cycle sequencing. Enter sample and customer names into the DNA database and schedule daily sequencing runs on ABI 3730 XL DNA analyzers. Perform basic data analysis using ABI Sequence Analysis Software and provide results to customers. Maintain precise records of all work. Collect DNA samples from five drop‑off locations on campus. Process samples using robotic liquid handlers (Beckman Coulter workstations) according to established protocols. Prepare buffers and solutions for various equipment and assays. Use a spectrophotometer or similar device to evaluate DNA quality and quantity, and prepare samples accordingly. Nanopore Sequencing Support (20%) Assist with preparing and performing quality control of Oxford Nanopore sequencing libraries according to established protocols. Prepare Nanopore flow cells for sequencing. Set up sequencing runs using Nanopore software. Initiate sequencing runs of experimental samples using MinION or similar devices. Perform data analysis according to documented data processing procedures. Maintain sequencing records containing operational parameters, customer and library preparation data, and other pertinent experimental information. Training & Laboratory Support (10%) Assist with training and supervision of student laboratory assistants under supervision. Assist staff and student employees with troubleshooting failed or poor sequencing results or sample issues. Assist student employees with setting up and organizing sequencing plates. Assist students and customers with technical aspects of pipetting, DNA preparation, reagent concentrations, primer use, and related laboratory procedures. Assist in updating and maintaining laboratory standard operating procedures and documentation. Equipment Maintenance & Troubleshooting (10%) Perform weekly restart of four 3730 XL DNA analyzers, three Beckman Coulter Biomek NX robots, and related computers. Clean ABI 3730 XL DNA analyzers and load new polymers regularly. Perform instrument calibrations and regular maintenance on DNA analyzers and automated liquid handlers. Troubleshoot equipment problems and communicate issues to relevant laboratory personnel. Assist in facilitating the laboratory transition to more digitized sample handling processes. Laboratory Operations & Inventory Management (10%) Assist in maintaining overall cleanliness and organization in the laboratory. Monitor supply levels of commonly used laboratory supplies, reagents, consumables, and ancillary materials. Procure laboratory supplies from approved sources as directed. Communicate with the director and/or staff regarding supply and inventory needs. Required Qualifications Must be a team player and adaptable to changes in the work environment. Knowledge of a wide variety of specialized laboratory equipment, including automated sequencers, thermal cyclers, and automated liquid handlers. Knowledge of laboratory safety procedures and requirements, as well as chemical disposal guidelines. Ability to work independently in an efficient manner and with attention to detail. Excellent interpersonal, organizational, and oral/written communication skills. Preferred Qualifications Experience in a medium to high‑throughput environment. Strong computer skills and general understanding of DNA sequencing and genotyping. Bioinformatics and programming experience. Education / Training Degree in Molecular Biology, Biochemistry, Chemistry, Physics, or related scientific field plus hands‑on research experience. Salary & Benefits For information on the comprehensive benefits package offered by the University, please visit the University of California's Compensation & Benefits website. Under California law, the University of California, Berkeley is required to provide a reasonable estimate of the compensation range for this role and should not offer a salary outside of the range posted in this job announcement. This range takes into account the wide range of factors that are considered in making compensation decisions, including but not limited to experience, skills, knowledge, abilities, education, licensure and certifications, analysis of internal equity, and other business and organizational needs. It is not typical for an individual to be offered a salary at or near the top of the range for a position. Salary offers are determined based on final candidate qualifications and experience. The budgeted hourly range that the University reasonably expects to pay for this position is $28.69 - $31.97, Step 1 - 6. This is a non‑exempt, bi‑weekly paid position. This is a full‑time (100% FTE, 40 hours/week) career position eligible for full UC benefits. Other Information This position is expected to work primarily on‑site due to the hands‑on laboratory and equipment responsibilities associated with the role. Working Days: Monday – Friday Working Hours: 7:30 AM – 4:00 PM Equal Employment Opportunity The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law. #J-18808-Ljbffr University of California-Berkeley